reference strain p aeruginosa atcc 27853  (ATCC)

Journal: Analytical Chemistry

Article Title: Test Strips Based on Gated Nanoporous Anodic Alumina for the Rapid and Accurate Detection of Pseudomonas aeruginosa in Clinical Samples

doi: 10.1021/acs.analchem.5c07621

Figure Lengend Snippet: Controlled release of RhB from the pores of the S3 material in TRIS buffer (pH 7.5) in the absence and presence of the purified phz A2 gene target sequences, R phz A2 , and the genomic DNA of P. aeruginosa ATCC 27853. (A) S3 in the presence/absence of R phz A2 . Red line indicates the intensity of RhB released in the presence of R phz A2 , whereas blue line indicates the intensity of RhB released in the absence of R phz A2 ( n = 3). (B) S3 support in the presence/absence of 2 ng μL –1 of genomic DNA of P. aeruginosa ATCC 27853. Red line indicates the intensity of RhB released in the presence of 2 ng μL –1 of genomic DNA of P. aeruginosa ATCC 27853, whereas blue line indicates the intensity of RhB released in the absence of genomic DNA of P. aeruginosa ( n = 3).

Article Snippet: Both phenotypes were successfully detected without significant differences; however, a slightly higher fluorescence signal was observed in the nonmucoid strain P. aeruginosa ATCC 27853.

Techniques: Purification

Journal: Analytical Chemistry

Article Title: Test Strips Based on Gated Nanoporous Anodic Alumina for the Rapid and Accurate Detection of Pseudomonas aeruginosa in Clinical Samples

doi: 10.1021/acs.analchem.5c07621

Figure Lengend Snippet: Response of S3 versus genomic DNA concentration of P. aeruginosa ATCC 27853 and versus other P. aeruginosa clinical strains and sensitivity to different bacterial concentrations of P. aeruginosa in TRIS buffer (pH 7.5). (A) Fluorescence from RhB released as a function of the concentration of genomic DNA of P. aeruginosa ATCC 27853. (B) Response of S3 in the presence of genomic DNA of different clinical strains of P. aeruginosa. (C) RhB release pattern from support S3 in the presence of different concentrations of P. aeruginosa (9.8 × 10 –3 to 8.7 × 10 3 CFU mL –1 ). (D) S3 response of the molecular recognition site and signal dependency: positive control (intact bacteria), resuspended cell pellet, filtered supernatant (containing eDNA) without/with DNase treatment. (E) Fluorescence RhB intensity according to the influence of the bacterial phenotype on biosensor response: nonmucoid ( P. aeruginosa ATCC 27853) and mucoid ( P. aeruginosa PFQ92) clinical strains at 10 3 CFU mL –1 .

Article Snippet: Both phenotypes were successfully detected without significant differences; however, a slightly higher fluorescence signal was observed in the nonmucoid strain P. aeruginosa ATCC 27853.

Techniques: Concentration Assay, Fluorescence, Positive Control, Bacteria

Journal: Analytical Chemistry

Article Title: Test Strips Based on Gated Nanoporous Anodic Alumina for the Rapid and Accurate Detection of Pseudomonas aeruginosa in Clinical Samples

doi: 10.1021/acs.analchem.5c07621

Figure Lengend Snippet: Intensity of RhB release in the presence of P. aeruginosa genomic DNA and human urine samples at 1 min in TRIS buffer (pH 7.5) using the S3-GF LFA biosensor. (A) Fluorescence of RhB released as a function of the increasing concentration of genomic DNA from P. aeruginosa ATCC 27853, along with the corresponding photographs recorded with a smartphone after a 1 min incubation period (see below). (B) Intensity of RhB released in the presence of human urine samples. The scatter plot plots the low fluorescence levels of the negative samples ( n = 6) (blue dots) compared to the higher fluorescence of the positive samples ( n = 11) (red dots). The fluorescence discrimination limit set is 0.184 fluorescence units.

Article Snippet: Both phenotypes were successfully detected without significant differences; however, a slightly higher fluorescence signal was observed in the nonmucoid strain P. aeruginosa ATCC 27853.

Techniques: Fluorescence, Concentration Assay, Incubation